The FUELSTAT® resinae PLUS DIESEL test utilises lateral flow technology and antibodies to H Res, bacteria and fungi which grow in diesel fuel.

 

Assay Description | Fuel Testing Company | Conidia Bioscience

 

The gold-labelled antibody is immobilised in the Release Pad under the sample well. The sample containing an unknown amount of contamination is added to the sample well and this re-hydrates, allowing the reagents to flow up (wicking) the membrane towards the absorbent pad. Any large particles in the sample, which may block the reaction, are blocked by the filtering action of the pad. During the wicking, the contamination in the sample will bind to the specific antibodies. As the liquid reaches the Test Line (T) any free gold-labelled antibodies will bind to the test line.

This means if more contamination is in the sample than the threshold engineered, there will be no antibodies to bind to the Test Line, no red line will appear, and this is a POSITIVE result. If the amount of contamination in the sample is lower than the threshold, there will be free antibodies to bind to the Test Line, a red line will appear, and this is a NEGLIGIBLE result. The quantities of materials immobilised in the device are engineered to provide results at the different thresholds of H Res, bacteria and fungi in each of the test devices on the Test Paddle.