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Take a sample from the fuel tank into a clean container. Allow the sample to “settle” and any water to accumulate at the bottom of the sampling container.

NOTE: When possible, test the water phase of the sample taken from the fuel tank. Testing the water phase will provide more accurate results than testing the fuel stage.

Testing Procedure (the same method is used for either test kit)

1. Unscrew and remove the top from the Sample Extraction bottle.  If there is 15ml of water in the sample, then add the water to the bottle up to the “Water Phase” line. If there is less than 15ml of   water in the sample, then add all the water and the fuel from the sample to the bottle, up to the “Fuel Phase” line

2. Replace the top and shake the bottle for 5 seconds.
3. Invert the bottle and allow the blue solution to settle completely into the dropper.
4. Remove the Test Paddle from the foil pouch.

NOTE: Do not touch the viewing windows on the test paddle. Touching the viewing windows can contaminate the test paddle. Inspect both viewing windows. There should be a faint blue line opposite the Control Line mark (C). This is used in the production process to line up the device correctly. It has no actual function in the test and will disappear when the sample moves up the wick. If any other visible lines show in a viewing window, then the paddle is contaminated.

5. Write the aircraft number, tank number aircraft cycles and hours onto the test paddle.

6. Remove the cap from the top of the Fuelstat® Sample Extraction Buffer bottle.

• Discard the first two drops from the bottle.
• Place 4 drops of the blue extraction liquid in each sample well. (The sample well will turn blue).

NOTE: The blue extraction liquid, NOT the fuel or water sample, must be placed in the sample well.

7. Keep the test paddle flat for at least 30 seconds.

8. Wait for 10 minutes.

• Make sure the Control Lines show after 10 minutes.
• If any Control Lines do not show, then the test is not valid.  Read within 30 minutes

Note the different strengths of the “C” and “T” lines. This is normal.

NOTES:
1. If the detection test shows microbial growth, then do the scheduled inspection test more often.
2. After a treatment with biocide, don’t test again until you fly at least 5 flights. This is to make sure that fuel treatment with biocide is fully removed from the fuel tanks before the next test.
3. If the biocide treatment is not effective using 1/3 fuel load, use a biocide treatment with a full fuel load and use the maximum soak time. The contamination may be towards the top of the tank.